Technical Data
4-Hydroxynonenal, Conjugated (4-HNE, HNE, 4-Hydroxy-2-nonenal)
4-Hydroxynonenal is a A 65 kD, highly reactive aldehyde released upon oxidation of omega-6-insaturated fatty acids, often by free radicals. HNE acts to spread and increase the initial effects of free radical events. It binds nucleic acids, phospholipids and sulfhydryl, histidine and lysine groups. These conjugates cause the cytotoxic effects (including cell death) that occur during oxidative stress due to hydrogen peroxide, superoxide, UV, heat and oxidant chemicals. HNE plays a role in the pathogenesis of diseases, stimulating fibrogenesis and inflammation. It is thought to act as a sensor of external stimuli, inducing the stress response by modulating membrane receptors such as Epidermal growth factor receptor or Fas.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 1:10,000
Western Blot: 1:3000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceGoat
Not Determined
4-Hydroxynonenal conjugate
Supplied as a liquid, 0.09% sodium azide
Recognizes 4-Hydroxynonenal.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Wu J, et al. Two dimensional blue native/SDS-PAGE to identify mitochondrial complex I subunits modified by 4-hydroxynonenal (HNE). 2015. Front. Physiol. 6:98. doi:10.3389/fphys.2015.00098.
Other References: 1. Boon, P. et al. (1999) Glutathione Conjugation of 4Hydroxytrans2,3nonenal in the Rat in Vivo, the Isolated Perfused Liver and Erythrocytes. Toxicol. Appl. Pharmacol. 159:21423. 2. Dwivedi S, et al. (2007) Role of 4hydroxynonenal and its metabolites in signaling. Redox. Rep. 12:410.