Technical Data
H6275-02
HNE (4-Hydroxy-2-nonenal, 4-Hydroxynonenal, 4-HNE)
Description:
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:10000
Western Blot: 1:3000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul4°C/-20°C (No Glycerol)Blue IceGoat
Concentration:
Not determined
Immunogen:
4hydroxynonenal conjugate
Purity:
Serum
Form
Supplied as a liquid, 0.09% sodium azide.
Specificity:
Recognizes 4HYDROXYNONENAL.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Boon, P. et al. (1999) Glutathione Conjugation of 4Hydroxytrans2,3nonenal in the Rat in Vivo, the Isolated Perfused Liver and Erythrocytes. Toxicol. Appl. Pharmacol. 159:21423. 2. Dwivedi S, et al. (2007) Role of 4hydroxynonenal and its metabolites in signaling. Redox. Rep. 12:410.