Technical Data
HNE (4-Hydroxy-2-nonenal)
HNE is a major product of endogenous lipid peroxidation. The w-6-family (linoleic and arachidonic acids) of polyunsaturated fatty acids may produce HNE as a result of free radical attack. HNE is a highly reactive compound and it can react with several functional groups on biological material, particularly sulfydryl groups to form thioester adduct and then hemiacetals. HNE may also react with histidine and lysine residues of proteins to form stable Michael addition-type of adducts. HNE-modified proteins may display an altered biological functions. Antibodies to HNE will help to visualize the HNE-adducts.

Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:1000-1:10,000
Western Blot: 1:500-1:1000
Immunohistochemistry: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
50ul-20CBlue IceRabbit
Not Determined
Free HNE conjugated to KLH.
Supplied as a liquid in PBS, 0.05% sodium azide.
Recognizes HNE (4-Hydroxy-2-nonenal).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Yoritaka, A., et al., Proc. Natl. Acad. Sci. 93: 2696-2701 (1996). 2. Uchida, K., Arch. Biochem. Biophys. 324: 241-248 (1995). 3. Quinn, M.T., et al., J. Leukocyte Biol. 57: 415-421 (1995). 4. Okamoto, K., Int. J. Cancer 58: 825-829 (1994). 5. Uchida, K., et al., Proc. Natl. Acad. Sci. 90: 8742-8746 (1993).