Technical Data
HNE (4-Hydroxy-2-nonenal)
HNE is a major product of endogenous lipid peroxidation. The w-6-family (linoleic and arachidonic acids) of polyunsaturated fatty acids may produce HNE as a result of free radical attack. HNE is a highly reactive compound and it can react with several functional groups on biological material, particularly sulfydryl groups to form thioester adduct and then hemiacetals. HNE may also react with histidine and lysine residues of proteins to form stable Michael addition-type of adducts. HNE-modified proteins may display altered biological functions. Antibodies to HNE will help to visualize the HNE-adducts.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
ELISA: 1:1000-1:10,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceGoat
Not determined
Free HNE (KLH).
Supplied as a lyophillized powder from 0.05% sodium azide.
Recognizes HNE (4-Hydroxy-2-nonenal).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Yoritaka, A., et al., 1996, Proc. Natl. Acad. Sci. 93: 2,696-2,701. 2. Uchida, K., 1995, Arch. Biochem. Biophys. 324: 241-248. 3. Quinn, M.T., et al., 1995, J. Leukocyte Biol. 57: 415-421. 4. Okamoto, K., 1994, Int. J. Cancer 58: 825-829. 5. Uchida, K., et al., 1993, Proc. Natl. Acad. Sci. 90: 8,742-8,746.