Technical Data
H6285-25D
HNRPK (Heterogeneous Nuclear Ribonucleoprotein K, Transformation Up-regulated Nuclear Protein, hnRNP K, TUNP, HNRNPK)
Description:
hnRNP K (Heterogeneous nuclear ribonucleoprotein K) is a pre-mRNA binding protein and a component of hnRNP complexes providing substrate for pre-mRNA processing before becoming translatable mRNAs in cytoplasm. This protein containing KH domains and is ubiquitously expressed in the cytoplasm and the nucleus with a predicted molecular weight approximately 51kD. hnRNP K expression and function are increased by epidermal growth factor (EGF). hnRNP K acts as transcriptional regulator and functions in nucleocytoplasmic shuttling, and is modified by phosphorylation (PKC delta, c-Src) and acetylation.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,kF45P9C7Affinity Purified
SizeStorageShippingSourceHost
25ug-20CBlue IceMouse
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide SVKQYSGKFF conjugated to Ovalbumin.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes hnRNPK. Species Crossreactivity: human, mouse, rat, rabbit and chicken.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Matunis MJ, et al. 1992. Mol. Cell. Biol. 12:164. 2. Dejgaard K, et al. 1994. J. Mol. Biol. 236:33. 3. Mandal M, et al. 2001. J. Biol. Chem. 276:9699