Technical Data
H6950-10
Homocysteine
Description:
Homocysteine a sulfur containing amino acid, is formed by the transmethylation of methionine, an essential amino acid. Homocysteine may be irreversibly metabolised to cystathionine by cystathionine synthetase. This reaction uses pyridoxal phosphate (Vitamin B6) as a co-enzyme (see figure). The enzyme cystathionine synthetase is deficient in homocysteinuria, a rare genetic disease associated with high blood levels of homocysteine and the development of premature atherosclerosis. This and the association of other rare enzyme deficiencies with homocysteinemia and premature atherosclerosis led McCully to formulate the homocysteine hypothesis of atherosclerosis (McCully KS, The homocysteine theory of atherosclerosis. Atherosclerosis. 1976; 20: 23-27)

Applications:
Suitable for use in ELISA. Other applications have not been tested.

Recommended Dilutions:
ELISA: 1:2500-1:5000
Optimal dilutions to be determined by the researcher.

Compound Crossreactivity Ratio:
Homocysteine-G-BSA 1
Cysteine-G-BSA 1:450
Homocysteic acid-G-BSA >1:100,000

G: Glutaraldehyde
BSA: Bovine Serum Albumin

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceRabbit
Concentration:
Not Determined
Immunogen:
Homocysteine-Glutaraldehyde conjugated to BSA
Purity:
Serum
Form
Supplied as a liquid in PBS, pH 7.1, 0.1% sodium azide.
Specificity:
Recognizes Homocysteine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.