Technical Data
H7021-82
Hormone Sensitive Lipase (HSL)
Description:
HSL (hormone-sensitive lipase) catalyzes the hydrolysis of triacylglycerol, the rate-limiting step in lipolysis. Lipolytic stimuli activate adenylyl cyclase and thus increase intracellular cAMP levels, which in turn activate protein kinase A (PKA). PKA phosphorylates HSL at Ser563, Ser659 and Ser660, which stimulates HSL activity. In contrast, AMPK phosphorylates HSL at Ser565, which reduces HSL phosphorylation at Ser563 by PKA and inhibits HSL activity. Recent work indicates that phosphorylation at Ser600 by p44/42 MAPKs also enhances the enzymatic activity of HSL.

Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000
Immunoprecipitation: 1:50
Immunofluorescence (IF-IC): 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceRabbit
Concentration:
Not determined.
Immunogen:
Synthetic peptide derived from the sequence of human HSL (KLH).
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Specificity:
Recognizes endogenous levels of total HSL protein. Species Crossreactivity: mouse. Species sequence homology: human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Degerman, E. et al. (1990) Proc. Natl. Acad. Sci. USA 87:533537. 2. Anthonsen, M.W. et al. (1998) J. Biol. Chem. 273:215221. 3. Garton, A.J. and Yeaman, S.J. (1990) Eur. J. Biochem. 191:245250. 4. Greenberg, A.S. et al. (2001) J. Biol. Chem. 276:4545645461.