Technical Data
HSL, phosphorylated, Ser853 (LIPE, Hormone-sensitive lipase)
HSL (Hormone-sensitive lipase) is a key enzyme in fatty acid mobilization, overall energy homeostasis, and possibly steroidogenesis. It is acutely controlled through reversible phosphorylation by catecholamines and insulin (1). HSL is the rate-limiting enzyme in hydrolysis of triglycerides in adipose tissue and of cholesteryl esters in steroidogenic tissues including the adrenals, ovaries, testes, and macrophages (2, 3). HSL activity has been positively correlated with free and esterified cholesterol ratios in seminiferous tubules (STf) and interstitial tissue (ITf) enriched fractions, but not with triglyceride levels, during testicular development. HSL is localized to elongated spermatids and Sertoli cells, where its distribution is stage-dependent, and within the cells lining the excurrent ducts of the testis. The HSL protein levels and enzymatic activity in ITf and STf were reported positively correlated with serum testosterone levels during development (4).LIPE, Hormone-sensitive lipase

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:50,000-200,000
Optimal dilutions to be determined by the researcher.

Positive Control:
Western Blot: Human skeletal muscle lysates

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
100ul-20°CBlue IceHumanRabbit
Not determined.
Phospho-specific peptide corresponding to residues surrounding Serine 853 of human HSL. Only detects HSL phosphorylated on Serine 853.
Supplied as a liquid in 50mM tris-glycine, pH7.4, 0.15M NaCl, 40% glycerol, 0.01% sodium azide, 0.05% BSA.
Recognizes human HSL at~83kD when phosphorylated at Ser853. Species Crossreactivity: mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Holm C, et al. Science 241(4872):1503-6, 1988 2. Li Z, et al. Genomics 24(2):259-65, 1994 3. Osuga J, et al. Proc Natl Acad Sci U S A. 97(2):787-92, 2000 4. Kabbaj O, et al Biology of Reproduction 65:601-612, 2001.