Technical Data
H7022-35B
HOX11 (T-cell Leukemia Homeobox Protein 1, Homeobox Protein Hox-11, TLX1, Proto-oncogene TCL-3, T-cell Leukemia/Lymphoma Protein 3, TCL3)
Description:
HOX11, also called homeobox11 or Tcl-3 is located at the 10q24 T-Cell translocation breakpoint region. It encodes a homeobox-domain containing protein, HOX11, containing a glycine and proline-rich amino terminus. HOX11 is required for maintenance of the developing spleen and cell survival. The HOX11protein binds to a specific DNA sequence and localizes to the cell nucleus. It transactivates transcription of a reporter gene linked to a cis-regulatory element, acting as a positive transcription activator. The HOX11 gene is highly expressed in T-ALLs as a result of a t (7:10) (q35;q24) or t(10:14)(q24;qll) translocation. The homeobox gene is deregulated upon translocation into the T-cell receptor locus and activates a specific subset of tumor-associated target genes. HOX 11 gene translocation has been attributed to T-cell leukemia and lymphoid neoplasias.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-3ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human, mouse, rat liver

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa306-318 (LQNLQPWSDDSTK) of human HOX11, GenBank no CAA08834.1.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity:
Recognizes human HOX11. Species Crossreactivity: mouse and rat. Species sequence homology: chimpanzee, canine, bovine, xenopus, chicken.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Brake,R.L., Kees,U.R. and Watt,P.M. A complex containing PBX2 contributes to activation of the proto-oncogene HOX11. Biochem. Biophys. Res. Commun. 294 (1), 23-34 (2002). 2. Cave,H., et al. Clinical significance of HOX11L2 expression linked to t(5;14)(q35;q32), of HOX11 expression, and of SIL-TAL fusion in childhood T-cell malignancies: results of EORTC studies 58881 and 58951 Blood 103 (2), 442-450 (2004). 3. Allen,T.D., Zhu,Y.X., Hawley,T.S. and Hawley,R.G. TALE homeoproteins as HOX11-interacting partners in T-cell leukemia. Leuk. Lymphoma 39 (3-4), 241-256 (2000).