Technical Data
Hox A5 (Homeobox A5, Homeobox Protein Hox-A5, HOXA5, HOX1, HOX1.3, Homeobox Protein Hox-1C, HOX1C, MGC9376)
In vertebrates, the genes encoding the class of transcription factors called homeobox genes are found in clusters named A, B, C, and D on four separate chromosomes. Expression of these proteins is spatially and temporally regulated during embryonic development. HOXA5 gene is part of the A cluster on chromosome 7 and encodes a DNA-binding transcription factor which may regulate gene expression, morphogenesis, and differentiation. Methylation of this gene may result in the loss of its expression and, since the encoded protein upregulates the tumor suppressor p53, this protein may play an important role in tumorigenesis.

Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceHumanRabbit
Not determined.
Synthetic peptide corresponding to aa182-199 (KLHISHDNIGGPEGKRAR) of human HOXA5 protein.
Supplied as a liquid, 0.025% sodium azide, 50% glycerol.
Recognizes human Hox A5. Species Crossreactivity: chicken, chimpanzee, canine, mouse, xenopus. Species sequence homology: axoloti, zebrafish.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Chen,H., Rubin,E., Zhang,H., Chung,S., Jie,C.C., Garrett,E., Biswal,S. and Sukumar,S. Identification of transcriptional targets of HOXA5. J. Biol. Chem. 280 (19), 19373-19380 (2005). 2. Stasinopoulos,I.A., Mironchik,Y., Raman,A., Wildes,F., Winnard,P. Jr. and Raman,V. HOXA5-twist interaction alters p53 homeostasis in breast cancer cells. J. Biol. Chem. 280 (3), 2294-2299 (2005). 3. Chen,H., Chung,S. and Sukumar,S. HOXA5-induced apoptosis in breast cancer cells is mediated by caspases 2 and 8. Mol. Cell. Biol. 24 (2), 924-935 (2004).