Technical Data
Hox B4 (Homeobox Protein B4, HOXB4, HOX-B4, Homeobox 2F, Hox 2F, HOX 2, Hox 2.6)
Homebox transcription factors (Hox) are encoded by highly conserved developmental genes that are involved embryonic and early hematopoietic development. Specifically, Homeobox B4 (HoxB4) is a transcription factor encoded by HOXB4 gene and it is involved in the developmental regulation of specific positional identities on the anterior-posterior axis of cells. Like all members of HOX family, HoXB4 is abundantly expressed in primitive hematopoietic stem cell (HSC), but then decline with lineage-specific terminal differentiation. In HSC, stem cell self-renewal activity is regulated by USF1/2 binding to HoxB4, where binding possibly favors stem cell self-renewal instead of cell differentiation. Jun-B and Fra-1 has been linked as key mediators during cell proliferation and differentiation induced by HoxB4, which leads to increase expression of Cyclin D1 and G1 shortening.

Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:32000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
Synthetic peptide with sequence C-EYSQSDYLPSDH, from the internal region of human Hox B4 (NP_076920.1).
Purified by affinity chromatography.
Supplied as a liquid in Tris, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Recognizes human Hox B4. Species sequence homology: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Chan KM, Bonde S, Klump H, Zavazava N. Hematopoiesis and immunity of HOXB4-transduced embryonic stem cell-derived hematopoietic progenitor cells. Blood. 2008 Mar 15;111(6):2953-61. PMID: 18056836