Technical Data
H7500-08
HSH2D (Hematopoietic SH2 Domain-containing Protein, Hematopoietic SH2 Protein, Adaptor in Lymphocytes of Unknown Function X ALX)
Description:
May be a modulator of the apoptotic response through its ability to affect mitochondrial stability By similarity. Adapter protein involved in tyrosine kinase and CD28 signaling. Seems to affect CD28-mediated activation of the RE/AP element of the interleukin-2 promoter.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:4000
Western Blot: 0.3-1ug/ml, observed in lysates of cell line K562 on ~37kD bands
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to C-HSHVGYTLSYKAQ, from human HSH2D, at the internal region of the protein (NP_116244.1).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.
Specificity:
Recognizes human HSH2D. Species sequence homology: Mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Shapiro MJ, Chen YY, Shapiro VS, The carboxyl-terminal segment of the adaptor protein ALX directs its nuclear export during T cell activation. The Journal of biological chemistry 2005 Nov 280(46):38242-6.