Technical Data
H7500-18A
HAX1 (HS1BP1, HCLS1-associated Protein X-1, HS1-associating Protein X-1, HS1-binding Protein 1)
Description:
HAX1 is known to associate with hematopoietic cell-specific Lyn substrate 1 (HS1), one of the substrates of receptor-coupled tyrosine kinases activated during clonal expansion and deletion in lymphoid cells. It also interacts with the product of the polycystic kidney disease 2 (PKD2) gene and with the F-actin-binding protein, cortactin. HAX1 is also reported to bind to hairpin structures in vimentin and DNA polymerase beta mRNAs, so may play a role in mRNA stability and transport. It may also function in promoting cell survival. Defects in HAX1 are the cause of autosomal recessive severe congenital neutropenia 3 (SCN3) also called Kostmann disease.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

TypeIsotypeCloneGrade
MabIgG2b,k10C311Affinity Purified
SizeStorageShippingSourceHost
100ul20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant human HAX1 (1-279aa) purified from E. coli
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes human HAX1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Klein C, et al., (2007) Nat Genet. 39(1):8692 2.Yin H, et al., (2001) Cytokine. 15(3):12237