Technical Data
H7500-50C
HS3ST4 (Heparan Sulfate Glucosamine 3-O-sulfotransferase 4, Heparan Sulfate D-glucosaminyl 3-O-sulfotransferase 4, 3-OST-4, Heparan Sulfate 3-O-sulfotransferase 4, h3-OST-4, 3OST4)
Description:
Heparan sulfate is a highly sulfated polysaccharide that can be found on the cell surface and within the extracellular matrix. It is typically covalently attached to the protein core of proteoglycans, such as syndecans and glypicans. Heparin, on the other hand, can be considered as a highly sulfated version of heparan sulfate that is predominantly found in mast cells. Both heparin and heparan sulfate contain disaccharide repeats of uronic acid and N-acetylglucosamine and are modified by the same sulfotransferases. The uronic acid residues can be sulfated at the 2-O position by heparan sulfate 2-O sulfotransferase (HS2ST). The N-acetylglucosamine residues can be sulfated at the N, 3-O, and 6-O positions by N-deacetylase/N-sulfotransferases (NDSTs), heparan sulfate 3-O sulfotransferases (HS3STs) and heparan sulfate 6-O sulfotransferases (HS6STs) respectively. There are seven HS3STs in the human genome. HS3ST4 and HS3ST2 are brain specific and may participate in HS-dependent neurobiologic events. HS3ST4 can generate tetrasulfated heparan sulfate disaccharide, the most highly sulfated sugar found in biological samples, and may have a role in assisting HSV-1 entry and spread. HS3ST4 is a Golgi resident type II membrane protein and has the longest proline rich stem region among all HS3STs.

Applications:
Suitable for use in Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 8-25ug/ml
Immunoprecipitation: 25ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG112K103Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml after reconstitution
Immunogen:
Recombinant corresponding to aa184-456 from human HS3ST4 expressed in CHO cell line.
Purity:
Purified by Protein A/G affinity chromatography.
Form
Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
Specificity:
Recognizes human HS3ST4. Does not react to recombinant human HS3ST3B1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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