Technical Data
H7520
HS6ST1 (Heparan-sulfate 6-O-sulfotransferase 1, DKFZp547H098, FLJ25392, Heparan-sulfate 6-O-sulfotransferase 1, HS6ST, HS6ST-1, MGC116899, MGC116901)
Description:
Heparan-sulfate 6-O-sulfotransferase 1 (HS6ST1) is one of three enzymes that catalyze sulfation at the 6-O position of N-acetylglucosamine residues in heparan sulfate. Heparan sulfate proteoglycans (HSPGs) interact with a variety of proteins, such as growth factors, protease inhibitors, cytokines, lipoprotein lipase and viral envelope proteins. HSPGs play roles in a wide variety of processes including cell growth, differentiation, and motility, blood coagulation, lipid metabolism, and viral infection. Within the lumenal domain, human and mouse HS6ST1 share 97% aa sequence.

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 25ug/ml. Detects HS6ST1 in paraffin-embedded normal and cancer human liver tissue sections.
Optimal dilutions to be determined by the researcher.

Hybridoma:
Mouse myeloma cells with B cells from mouse immunized with purified, CHO cell-derived recombinant human HS6ST1.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2A9L832Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml (after reconstitution)
Immunogen:
Recombinant protein corresponding to aa38-411 from human HS6ST1, CHO derived (Accession# O60243).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Specificity:
Recognizes human HS6ST1. Species sequence homology: mouse 97%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.