Technical Data
H7981-02
Hyaluronidase 3 (Hyaluronoglucosaminidase 3, Hyal 3)
Description:
Hyaluronidases (HAases)1 are a family of enzymes that are crucial for the spread of bacterial infections, toxins present in various venoms, and possibly, cancer progression.1-6 In humans, six HAase genes have been identified. With the possible exception of HYAL4 and HYALP1, all other HAases are thought to degrade hyaluronic acid (HA).7 HA is a high molecular mass nonsulfated glycosaminoglycan made up of repeating disaccharide units, D-glucuronic acid, and N-acetyl-D-glucosamine. HA is present in body fluids, tissues, and the extracellular matrix of vertebrate tissues.8-10 It keeps tissues hydrated, and maintains osmotic balance, and cartilage integrity.8,9 HA also actively regulates cell adhesion, migration, and proliferation by interacting with specific cell surface receptors such as CD44 and RHAMM.11 The concentrations of HA are elevated in several inflammatory diseases, and various carcinomas such as bladder, prostate, breast, lung, and colon.8,12-19 HYAL3 transcripts have been detected in kidney, brain and liver tissues.20 HYAL3 has a classical TIM barrel topology, where the catalytic site corresponds to Asp127 and Glu129, respectively. Several mRNA splice variants of HYAL3 expressed in bladder and prostate cancer cells and tissues have been identified and characterized in terms of their protein product and HAase activity.21 A 30-aa region that is crucial for the HAase activity of HYAL3 protein was also identified.21

Applications:
Suitable for use in Western Blot, Immunoprecipitation. Other applications not tested.
Recommended Dilution:
Western Blotting: 1-3ug/ml
Immunoprecipitation: 5ug/reaction
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.


TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRabbit
Concentration:
0.25mg/ml
Immunogen:
Synthetic peptide derived from an internal region of the mouse Hyal3 (hyaluronidase-3, hyaluronoglucosaminidase 3) protein.
Purity:
Epitope-affinity purified from rabbit antiserum.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Reacts with the mouse Hyal3 protein. On Western blots of cells overexpressing Hyal3, the target band is identified at ~50kD. Reactivity has been confirmed with mouse Hyal3-transfected BHK cell lysates.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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2. Gmachl M and Kreil G. PNAS 90:3569-3573,1993.
3. Henrissat B. Biochem J 280(2):309-316,1991.
4. Henrissat B and Bairoch A. Biochem J 316(2): 695-696,1996.
5. Roden L, et al. Ciba Found Symp 143:281-285,1989.
6. Tu AT and Hendon RR. Comp Biochem Physiol B 76:377-383,1983.
7. Csoka AB, et al. Matrix Biol 20:499-508,2001.
8. Delpech B, et al. J Intern Med 242:41-48,1997.
9. Laurent TC and Fraser JR. Faseb J 6:2397-404,1992.
10. Tammi MI, et al. J Biol Chem 277:4581-4584,2002.
11. Turley EA, et al. J Biol Chem 277:4589-4592,2002.
12. Ropponen K, et al. Cancer Res 58:342-347,1998.
13. Toole BP, et al. J Biol Chem 277:4593-4596,2002.
14. Auvinen P, et al. Am J Pathol 156:529-536,2000.
15. Lokeshwar VB and Block NL Urol Clin North Am 27:53-61,2000.
16. Lokeshwar VB, et al. J Urol 163:348-356,2000.
17. Lokeshwar VB, et al. J Biol Chem 276:11922-11932,2001.
18. Pirinen R, et al. Int J Cancer 95:12-7,2001.
19. Setala LP, et al. Br J Cancer 79:1133-1138,1999.
20. Triggs-Raine B, et al. PNAS 96:6296-6300,1999.
21. Lokeshwar VB, et al. J Biol Chem 277:33654-33663,2002.