Technical Data
H8010
HRAS (HRAS1, H-Ras-1, c-H-ras, C-Ha-Ras1, Ha-Ras, GTPase HRas, C-Bas/Has, CTLO, HAMSV, H-RASIDX, K-Ras, p21ras, RASH1, Transforming Protein p21)
Description:
HRAS belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. These proteins function in signal transduction pathways. They can bind GTP and GDP, and they have intrinsic GTPase activity. HRAS undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in the HRAS gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide selected from the Center region of human HRAS (KLH).
Purity:
Purified by ammonium sulfate precipitation.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human HRAS.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Winter-Vann,A.M., Proc. Natl. Acad. Sci. USA 100(11), 6529-6534 (2003). 2.Coats,S.G., Biochemistry 38(39), 12926-12934 (1999). 3.Sakai,E., Int. J. Cancer 52(6), 867-872 (1992).