		Technical Data
H9042-24	8-Hydroxy-2’-deoxyguanosine (8-oxo-dG)

Description:
A marker compound indicative of DNA damage associated with mutagenesis and carcinogenesis. Can induce differentiation of Friend murine erythroleukemia cells in vitro.

Applications:
Suitable for use in ELISA and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunocytochemistry: 1:250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2b	9i232	Purified

Size		Storage	Shipping	Source	Host
50ul		-20°C	Blue Ice		Mouse

Concentration:

As reported

Immunogen:

Synthetic peptide corresponding to 8-oxo-dG.

Purity:

Purified.

Form

Supplied as a liquid in PBS, 0.01% sodium azide.

Specificity:

Recognizes 8-hydroxy-2'- deoxyguanosine within DNA in H2O2-treated cells.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Soultanakis RP, Melamede RJ, Bespalov IA, Wallace SS, Beckman KB, Ames BN, Taatjes DJ, Janssen-Heininger YMW. (2000) Flourescence detection of 8-oxoguanine in nuclear and mitochondrial DNA of cultured cells using a recombinant Fab and confocal scanning laser microscopy. Free Rad Biol Med28:987-998.