Technical Data
H9100
3-Hydroxykynurenine (3-OHKYN)
Description:
3-hydroxykynurenine, a chromophore metabolite of tryptophan, acts as UV protectant and an antioxidant in vertebrate lens. This compound reacts with proteins to induce cross-linking and chromophore adducts.

Applications:
Suitable for use in ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (paraffin) and Western Blot, Other applications not tested.

Recommended Dilution:
Immunocytochemistry/Immunofluorescence: 1:100-1:200
Western Blot: 2ug/ml. Band at ~66kD observed for 3-OHKYN-modifed BSA.
Optimal dilutions to be determined by the researcher.

Cellular Localization:
Cytoplasmic

Positive Control:
3-OHKYN modified BSA

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k6k292Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceMouse
Concentration:
~4mg/ml
Immunogen:
3-OHKYN modified KLH
Purity:
Purified by Protein G affinity chromatography
Form
Supplied as a liquidin Tris-glycine, 150mM sodium chloride, 0.05% sodium azide
Specificity:
Specific for 3-hydroxykynurenine modifications on N-alpha-acetyl lysine, N-alpha-acetyl histidine, N-alpha-acetyl arginine, and N-alpha-acetyl cysteine. It does not recognize the transaminase enzyme involved in the degradation of tryptophan. Reacts with 3-OHKYN modifications in human proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Staniszewska, MM and Nagaraj, RH. 3-hydroxykynuenine-mediated modification of human lens proteins. JBC. 280 (23): 22154-22164, 2005