Technical Data
Hydroxyoctadecadienoic acid (HODE) 9()
Linoleic acid, the predominant polyunsaturated fatty acid (PUFA) in the human diet, can be metabolized by cyclooxygenase, lipoxygenase and P450 enzymes. The hydroxyoctadecadienoic acid (HODE) derivatives of linoleic acid, 9(R)-HODE, 9(S)-HODE and 13(S)-HODE, are the most widely distributed of the known linoleic acid metabolites.

These compounds exhibit interesting biologic activities, including the regulation of platelet function, maintenance of vascular thromboresistance and transduction of the cellular responses to certain growth factors. HODE derivatives may also influence certain pathological states including, psoriasis, the development of atherosclerosis and the development of cancer.

Suitable for use in RIA and ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 2ug/well
RIA: 2ug/well
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul-20CBlue IceGoat
9(S)-HODE; 9(R)-HODE.
Purified by ammonium sulfate precipitation.
Supplied as a liquid in PBS.
Recognizes 9()-HODE. Crossreactivity: 9()-HODE: 100.0%, 9(S)-HODE: 100.0%, 9(R)-HODE: 100.0%, 13(S)-HODE: 1.2%, 13(R)-HODE: 1.2%, 9-oxo-ODE: 1.2%, 13-oxo-ODE: 2.4%, 11(S)-HETE: 0.0%, 15(S)-HETE: 0.0%, Linoleic Acid: 0.0%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Spindler, S.A., Reddy, R.G., Biomedical Products, July 1997, 2. Spindler, S.A., Clark, K.S., Callewaert, D.M., Reddy, R.G., Biochem. Biophys. Res. Commun., 218 : 187-191 (1996).