Technical Data
H9118-05
6-Hydroxytryptamine, Conjugated
Description:
Applications: Suitable for use in ELISA, Immunohistochemistry. Other applications not tested.

Specificity
Using a conjugate 6-hydroxytryptamine-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds:

Compounds Cross-reactivity ratio(a)
6-Hydroxytryptamine-G-BSA 1
5 Hydroxytryptamine-G-BSA 1/9,000
Tryptamine-G-BSA 1/>50,000
5-Methoxytryptamine-G-BSA 1/>50,000

(a) : 6-Hydroxytryptamine-G-BSA concentration/unconjugated or conjugated indolealkylamine concentration at half displacement.
G = Glutaraldehyde, BSA = bovine serum albumin

Recommended dilution
The antiserum was tested using the free floating PAP technique on rat raphe nuclei. The anti-conjugated 6-hydroxytryptamine antibodies stains well between a 1/2,000-1/5,000 dilution.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
50ug-20°CBlue IceRabbit
Concentration:
~1mg/ml
Immunogen:
6-Hydroxytryptamine-Glutaraldehyde-BSA
Purity:
Antiserum previously preabsobed on protein carriers, and purified by ammonium sulfate precipitation and gel filtration.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added.
Specificity:
Recognizes protein-conjugated Hydroxytryptamine,6-. See additional crossreactivity data.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.