Technical Data
Hygromycin B 50mg/ml Solution
Biochemicals Storage: 4CShipping: Blue Ice
Hygromycin B is an aminoglycosidic antibiotic which inhibits protein synthesis in bacteria, fungi and mammalian cells and is used to select prokaryotic and eukaryotic cells that have been transfected with the Hygromycin gene (hyg, hph, HMA, HM+).

Source: Streptomyces hygroscopicus

Purity (HPLC): 76%

Bioassay: 300-500units/mg solution

Density: 1.13g/ml

Concentration: 50mg Hygromycin B/ml

Form: Supplied as a liquid. Prepared with RNase, DNase-free, molecular biology grade water (W0900). Sterile-filtered (0.2um).

Recommended Concentration Range: 25-1500ug/ml
E.coli JM 83: 25-200ug/ml
Human: 50-400ug/ml
Saccharomyces cerevisiae: 200ug/ml
Chicken: 1500ug/ml
Tobacco: 20-200ug/ml

Storage and Stability:
May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Kojic, M. and Holloman, W.K. (2000) Can J Microbiol. 46:333-338. 1. Hamada, W., et al. 1994. Curr. Genetics 26, 251. 2. Buchschacher, G.L., Jr., and Panganiban, A.T. 1992. J. Virol. 66, 2731. 3. Gaken, J., et al. 1992. Biotechniques 13, 32. 4. Ma, H., et al. 1992. Gene 117, 161. 5. Lama, J., and Carrasco, L. 1992. J. Biol. Chem. 267, 15932. 6. Norman, J.A., et al. 1992. Mol. Pharmacol. 41, 53. 7. McIntyre, G., et al. 1992. Adv. Exp. Med. Biol. 276, 67. 8. Dale, E., and Ow, D. 1991. Proc. Natl. Acad. Sci. USA 88, 10558.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.