Technical Data
Hypoxia Inducible Factor 3a (Hypoxia Inducible Factor 3 alpha, Hypoxia Inducible Factor 3 alpha Subunit, Hypoxia Inducible Factor Three alpha, HIF-3a, HIF3A, HIF3 alpha, HIF-3A4, Inhibitory PAS Domain Protein, IPAS, MOP7, PASD7)
Hypoxia-inducible factor (HIF) is one of the most important factors in the cellular response to hypoxia, transcriptionally activating genes encoding proteins that mediate adaptive responses to reduced oxygen availability. HIF is a heterodimer consisting of one of three subunits, HIF1 alpha, HIF2 alpha, or HIF3 alpha. HIF target genes play critical roles in metabolism, angiogenesis, cell proliferation and cell survival. HIF3 alpha protein is one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha 3 subunit lacks the transactivation domain found in factors containing either the alpha 1 or alpha 2 subunits. HIF3 alpha may be a marker for tumor growth and angiogenesis.

Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Positive Control: Cos7 CoCl2-treated lysate

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 3 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul-20CBlue IceHumanRabbit
A synthetic peptide made to an internal region of the human HIF-3 alpha (within residues 250-350). [Swiss-Prot# Q66K72].
Supplied as a liquid in PBS, pH 7.2.
This antibody is specific for HIF-3 alpha. Species Crossreactivity: Recognizes human and mouse HIF-3 alpha.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Gu YZ, Moran SM, Hogenesch JB, Wartman L, Bradfield CA. Molecular characterization and chromosomal localization of a third alpha-class hypoxia inducible factor subunit, HIF3 alpha. Gene Expr. 1998;7(3):205-13.