Technical Data
H9810-05G
HIF3A (Hypoxia-inducible Factor 3-alpha, HIF-3-alpha, HIF3-alpha, Basic-helix-loop-helix-PAS Protein MOP7, Class E Basic Helix-loop-helix Protein 17, bHLHe17, HIF3-alpha-1, Inhibitory PAS Domain Protein, IPAS, Member of PAS Protein 7, PAS Domain-containin
Description:
Hypoxia-inducible factor (HIF) is one of the most important factors in the cellular response to hypoxia, transcriptionally activating genes encoding proteins that mediate adaptive responses to reduced oxygen availability. HIF is a heterodimer consisting of one of three subunits, HIF1 alpha, HIF2 alpha, or HIF3 alpha. HIF target genes play critical roles in metabolism, angiogenesis, cell proliferation and cell survival. HIF3 alpha protein is one of several alpha/beta-subunit heterodimeric transcription factors that regulate many adaptive responses to low oxygen tension (hypoxia). The alpha 3 subunit lacks the transactivation domain found in factors containing either the alpha 1 or alpha 2 subunits. HIF3 alpha may be a marker for tumor growth and angiogenesis.

Applications:
Suitable for use in ELISA, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:100-1:8000
ELISA: 1:14000-80000
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceMouseRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to aa581-592 of mouse HIF3A (KLH).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.
Specificity:
Recognizes mouse HIF3A. Species sequence homology: Human and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.