Technical Data
Ice Protease Activating Factor (IPAF, Caspase recruitment domain containing protein 12, CARD12, CARD LRR and NACHT Containing Protein 1, CLAN1, CLANA, CLANB, CLANC, Clan Protein, CLR2.1, NLR Family CARD Domain Containing 4, NLRC4)
Ipaf (also known as Clan/CARD12) is a CARD domain containing protein. CARD (caspase-associated recruitment domain) proteins are key regulators of cell death, cell survival and cytokine production (reviewed in Damiano and Reed, 2004). In general CARD proteins are implicated in host defense against infection, environmental stress or cellular damage. CARD domains are found in the N-terminal pro-domains of certain caspases, a family of apoptotic and pro-inflammatory proteases, as well as in a diversity of other proteins including Ipaf/Clan/CARD12. CARD domains are homotypic protein interaction motifs that enable networks of proteins to communicate via CARD-CARD interactions. There are at least three major signaling pathways in which CARD proteins act: (1) Regulation of caspase activation in the context of apoptosis (2) Regulation of caspase activation in the context of inflammation (3) Regaultion of NF-kB activation in the context of innate or adaptive immune responses.

Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin): 1:1000-1:5000
Immunohistochemistry (frozen): 1:10-1:2000
Immunoprecipitation: 1:50-1:200
Western Blot: 1:1000-1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
50ul-20CBlue IceRabbit
Not determined
Synthetic peptide corresponding to aa637-654, EEAPETYIPSRAVSLFFN of human Ice Protease Activating Factor (NP_067032.3).
Supplied as a liquid, 0.05% sodium azide.
Recognizes human IPAF. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.