Technical Data
I0650-01
ICOS (CD278, Inducible T cell co stimulator, Activation inducible lymphocyte immunomediatory molecule, AILIM, MGC39850)
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Technical Data |
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I0650-01 |
ICOS (CD278, Inducible T cell co stimulator, Activation inducible lymphocyte immunomediatory molecule, AILIM, MGC39850) |
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Description: The inducible costimulator (ICOS, T cell activation molecule H4) is similar to human CD28 (24% homology), and plays an analogous role in the T cell activation process. Secondary signaling through CD28 or ICOS results in discrete cytokine secretion profiles by the activated T cells(1)/ Signaling by either molecule is effectively down regulated by CD152 (CTLA-4) engagement(2). Human GL50 is a member of the B7 family sharing ~20% homology with CD80 (B7-1) and CD86 (B7-2), and has been shown to be the ligand for ICOS.3 Two RNA splice variants exist for this molecule, differing only in the cytoplasmic domain. Applications: Suitable for use in ELISA and Flow Cytometry and Functional application. Other applications not tested. Recommended Dilution: Functional Application: Antibody blocks binding of Recombinant GL50-muIg to ICOS Flow Cytometry: 1ug/ml. 5x10e5 human HPB-MLT cells were washed, incubated for 45 min. on ice with 80ul of I0650-01 at 1ug/ml. Cells were washed twice and incubated appropriate secondary antibody, after which they were washed twice, fixed and analyzed by FC. Cells stained positive with a mean shift of 1.3 log10 fluorescent units when compared to isotype control mouse IgG. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
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