Technical Data
ICOS (CD278, Inducible T cell co stimulator, Activation inducible lymphocyte immunomediatory molecule, AILIM, MGC39850)
The inducible costimulator (ICOS, T cell activation molecule H4) is similar to human CD28 (24% homology), and plays an analogous role in the T cell activation process. Secondary signaling through CD28 or ICOS results in discrete cytokine secretion profiles by the activated T cells(1)/ Signaling by either molecule is effectively down regulated by CD152 (CTLA-4) engagement(2). Human GL50 is a member of the B7 family sharing ~20% homology with CD80 (B7-1) and CD86 (B7-2), and has been shown to be the ligand for ICOS.3 Two RNA splice variants exist for this molecule, differing only in the cytoplasmic domain.

Suitable for use in ELISA, Flow Cytometry and Functional application. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1ug/ml. 5x10e5 human HPB-MLT cells were washed, incubated for 45 minutes on ice with 80ul of
I0650-01 at 1ug/ml. Cells were washed twice and incubated with IgG (FITC) goat anti-mouse secondary antibody, after which they were washed twice, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.63 log10 fluorescent units when compared to isotype control mouse IgGI antibody.
Functional Application: Antibody blocks binding of Recombinant GL50-muIg to HPB-MLT cells.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG1,k7H152Affinity Purified
100ug-20CBlue IceHumanMouse
Human HPB-MLT cells, human ICOS-muIg fusion protein
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, pH 7.5, 0.5 mg/ml gentamicin sulfate
Recognizes human recombinant and cell surface ICOS.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Beier, K.C., R.A. Kroczek, et al. 2000, Eur J Immunol. 30:3707-3717. 2. Riley, J.L., C.H. June, et al. 2001, J. Immunol.166: 4943-4948. 3. Ling, V., M. Collins, et al. 2000, J. Immunol.164: 1653-1657. 4. Ling, V., M. Collins, et al. 2001, J. Immunol.166: 7300-7308.