Technical Data
I0650-01
ICOS (CD278, Inducible T cell co stimulator, Activation inducible lymphocyte immunomediatory molecule, AILIM, MGC39850)
Description:
The inducible costimulator (ICOS, T cell activation molecule H4) is similar to human CD28 (24% homology), and plays an analogous role in the T cell activation process. Secondary signaling through CD28 or ICOS results in discrete cytokine secretion profiles by the activated T cells(1)/ Signaling by either molecule is effectively down regulated by CD152 (CTLA-4) engagement(2). Human GL50 is a member of the B7 family sharing ~20% homology with CD80 (B7-1) and CD86 (B7-2), and has been shown to be the ligand for ICOS.3 Two RNA splice variants exist for this molecule, differing only in the cytoplasmic domain.

Applications:
Suitable for use in ELISA, Flow Cytometry and Functional application. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1ug/ml. 5x10e5 human HPB-MLT cells were washed, incubated for 45 minutes on ice with 80ul of
I0650-01 at 1ug/ml. Cells were washed twice and incubated with IgG (FITC) goat anti-mouse secondary antibody, after which they were washed twice, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.63 log10 fluorescent units when compared to isotype control mouse IgGI antibody.
Functional Application: Antibody blocks binding of Recombinant GL50-muIg to HPB-MLT cells.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k7H152Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human HPB-MLT cells, human ICOS-muIg fusion protein
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.5, 0.5 mg/ml gentamicin sulfate
Specificity:
Recognizes human recombinant and cell surface ICOS.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Beier, K.C., R.A. Kroczek, et al. 2000, Eur J Immunol. 30:3707-3717. 2. Riley, J.L., C.H. June, et al. 2001, J. Immunol.166: 4943-4948. 3. Ling, V., M. Collins, et al. 2000, J. Immunol.164: 1653-1657. 4. Ling, V., M. Collins, et al. 2001, J. Immunol.166: 7300-7308.