Technical Data
ICOS (CD278, Inducible T cell co stimulator, Activation inducible lymphocyte immunomediatory molecule, AILIM, MGC39850)
The inducible costimulator (ICOS, T cell activation molecule H4) is similar to human CD28 (24% homology), and plays an analogous role in the T cell activation process. Secondary signaling through CD28 or ICOS results in discrete cytokine secretion profiles by the activated T cells(1)/ Signaling by either molecule is effectively down regulated by CD152 (CTLA-4) engagement(2). Human GL50 is a member of the B7 family sharing ~20% homology with CD80 (B7-1) and CD86 (B7-2), and has been shown to be the ligand for ICOS.3 Two RNA splice variants exist for this molecule, differing only in the cytoplasmic domain.

Suitable for use in ELISA and Flow Cytometry and Functional application. Other applications not tested.

Recommended Dilution:
Functional Application: Antibody blocks binding of Recombinant GL50-muIg to ICOS
Flow Cytometry: 1ug/ml. 5x10e5 human HPB-MLT cells were washed, incubated for 45 min. on ice with 80ul of
I0650-01 at 1ug/ml. Cells were washed twice and incubated appropriate secondary antibody, after which they were washed twice, fixed and analyzed by FC. Cells stained positive with a mean shift of 1.3 log10 fluorescent units when compared to isotype control mouse IgG.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG1,k7H152Affinity Purified
100ug4C (-20C Glycerol)Blue IceHumanMouse
Human HPB-MLT cells, recombinant human ICOS-muIg
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, pH 7.5, 0.5 mg/ml Gentamicin Sulfate.
Binds to recombinant and cell surface ICOS.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1) Beier, K.C., R.A. Kroczek, et al. 2000, Eur J Immunol. 30(12):3707-3717.
2) Riley, J.L., C.H. June, et al. 2001, J. Immunol.166 : 4943-4948.
3) Ling, V., M. Collins, et al. 2000, J. Immunol.164: 1653-1657.
4) Ling, V., M. Collins, et al. 2001, J. Immunol.166: 7300-7308.