Technical Data
ICOS (CD278, Inducible T cell co stimulator, Activation inducible lymphocyte immunomediatory molecule, AILIM, MGC39850) (Biotin)
The inducible costimulator (ICOS, T cell activation molecule H4) is similar to human CD28 (24% homology), and plays an analogous role in the T cell activation process. Secondary signaling through CD28 or ICOS results in discrete cytokine secretion profiles by the activated T cells(1). Signaling by either molecule is effectively down regulated by CD152 (CTLA-4) engagement(2). Human GL50 is a member of the B7 family sharing ~20% homology with CD80 (B7-1) and CD86 (B7-2), and has been shown to be the ligand for ICOS(3). Two RNA splice variants exist for this molecule, differing only in the cytoplasmic domain.

Suitable for use in ELISA, Flow Cytometry and Functional Studies. Other applications not tested.

Recommended Dilution:
Functional Application: Blocks binding of recombinant GL50-muIg to HPB-MLT cells.
Flow cytometry: 1ug/ml
Optimal dilutions to be determined by the researcher.

1. Five x 10e5 cultured human HPB-MLT cells were washed and incubated 45 minutes on ice with 80ul of I0650-01A at 1ug/ml.
2. Cells were washed twice and incubated with Streptavidin(PE) (S7973-88P) after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.38 log10 fluorescent units when compared to a Mouse IgG1(Biotin) negative control at a similar concentration.
3. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml anti-CD278 antibody (I0650-01).

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG1,k7H152Affinity Purified
100ug-20CBlue IceHumanMouse
Human HPB-MLT cells, recombinant human ICOS-muIg.
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.
Recognizes human ICOS. Binds to recombinant ICOS-muIg in ELISA, and to the cell surface of HPB-MLT cells in flow cytometry.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1) Beier, K.C., R.A. Kroczek, et al. 2000, Eur J Immunol. 30(12):3707-3717.
2) Riley, J.L., C.H. June, et al. 2001, J. Immunol.166 : 4943-4948.
3) Ling, V., M. Collins, et al. 2000, J. Immunol.164: 1653-1657.
4) Ling, V., M. Collins, et al. 2001, J. Immunol.166: 7300-7308.