Technical Data
I0650-01D
ICOS Ligand (CD275, Inducible T cell co-stimulator ligand, B7-H2, B7 homolog 2, B7-like protein Gl50, B7 related protein 1, B7H2, B7RP-1, B7RP1, GL50, ICOS LG , ICOSLG, KIAA0653, LICOS, Transmembrane protein B7 H2 ICOS ligand)
Description:
The human ICOS Ligand (B7-H2) is a 60kD member of the B7 family of immune costimulatory proteins, which includes B7-1, B7-2, B7-H1 (PD-L1), PD-L2 and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily, the extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Family members share about 20-25% aa identity. Within the extracellular domain, human B7-H2 shares 49% and 54% aa sequence identity with mouse and rat B7-H2, respectively. The ligand for ICOS is a member of the CD28 family of costimulatory receptors. Human B7-H2 is a 309 aa protein with a putative 18aa signal peptide, a 239aa extracellular domain, an 18aa transmembrane region and a 33aa cytoplasmic domain. Human B7-H2 is expressed constitutively on resting B cells, dendritic cells, and at low levels on monocytes. The B7-H2/ICOS interaction appears to play roles in T cell dependent B cell activation and Th differentiation.

Applications:
Suitable for use in Neutralization, Flow Cytometry, Western Blot and Direct ELISA. Other applications not tested.

Recommended Dilutions:
Neutralization: Measured by its ability to neutralize B7-H2-induced proliferation in PHA-activated human T cells. The Neutralization Dose (ND50) is typically 1-4ug/ml in the presence of 3ug/ml Recombinant Human B7-H2 Fc Chimera and 20ng/ml Human CD3-epsilon Mab.
Flow Cytometry: 2.5ug labels 10e6 U937 cells
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. 
TypeIsotypeCloneGrade
MabIgG2b8H104Affinity Purified
SizeStorageShippingSourceHost
500ug-20°CBlue IceHumanMouse
Concentration:
~0.5mg/ml (after reconstitution)
Immunogen:
Recombinant protein corresponding to Asp19-Ser258 from human ICOS Ligand expressed in NSO cells (O75144).
Purity:
Purified by Protein A affinity chromatography. Endotoxin: <0.1EU/ug (LAL).
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 1ml sterile PBS.
Specificity:
Recognizes human ICOS Ligand (B7-H2). Does not crossreact with recombinant human B7-1, B7-2, B7-H1, B7-H3 or recombinant mouse B7-H2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Coyle, A.J. and J.C. Gutierrez­Ramos (2001) Nat. Immunol. 2:203. 2. Ling, V. et al. (2000) J. Immunol. 164:1653. 3. Wang, S. et al. (2000) Blood 96:2808. 4. Brodie, D. et al. (2000) Curr. Biol. 10:333. 5. Mages, H.W. et al. (2000) Eur. J. Immunol. 30:1040. 6. Swallow, M.M. et al. (1999) Immunity 11:423. 7. Yoshinaga, S.K. et al. (1999) Nature 402:827.