		Technical Data
I0651-02	B7-H4 (B7x) (FITC)

Description:
B7-H4, also known as B7x, is a costimulatory protein which is reported to function as a negative regulator of T-cell mediated immunity. Although B7-H4 binds an unknown receptor, it is thought to deliver an inhibitory signal to T-cells preventing their proliferation, cell cycle progression and interleukin-2 production. B7-H4 deficient mice are only minimally affected; suggesting B7-H4 is important in the fine tuning of the T-cell mediated immune response.B7-H4 is expressed on activated T-cells, B-cells, monocytes and dendritic cells. Aberrant expression has been associated with cancers of the lung, breast and ovary in humans. B7-H4 could be a useful prognostic marker in Renal Cell Carcinoma (RCC).

Applications:
Suitable for use in Flow Cytometry. Other applications have not been tested.

Recommended Dilutions:
Flow Cytometry: Neat-1:5. Use 10ul to label 1x10e6 cells in 100ul.
Optimal dilutions to be determined by the researcher.

Recommended Negative Control:
I1904-78R3: IgG1 Murine Negative Control (FITC)

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.

Type	Isotype	Clone	Grade
Mab	IgG1	9A174	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~0.1mg/ml

Immunogen:

Human B7-H4

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).

Specificity:

Recognizes human B7-H4.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Krambeck, A.E. et al. (2006) B7-H4 expression in renal cell carcinoma and tumor vasculature: associations with cancer progression and survival. Proc. Natl. Acad. Sci. USA. 103: 10391-10396 2. Van de Ven R et al., Blood 118:2502-10 (2011)