Suitable for use in Immunocytochemistry and Western Blot. Other applications have not been tested.
Western Blot: 1:200
Optimal dilution to determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|Synthetic peptide corresponding to aa 17-31 (D17LKEEKDINNNVKKT31) of mouse inducible nitric oxide synthase (iNOS; NOS II)|
|Liquid. Purified IgG in PBS containing 1mg/ml BSA and 0.05% sodium azide|
|Recognizes human, mouse and rat iNOS (NOS II). Detects a band of ~135kDa by Western blot. Does not cross-react with eNOS (NOS III) or nNOS (NOS I).|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
Upregulation of nitric oxide synthase in mice with severe hypoxia-induced pulmonary hypertension: K.A. Fagan, et al.; Respir. Res. 2, 306 (2001) |