Description:
Applications: Suitable for use in Immunocytochemistry and Western Blot. Other applications have not been tested.
Recommended Dilution: Immunocytochemistry: 1:50 Western Blot: 1:200 Optimal dilution to determined by the researcher.
Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|
| Type | Isotype | Clone | Grade |
| Pab | | | Purified |
|
| Size | | Storage | Shipping | Source | Host |
| 200ul | | -20°C | Blue Ice | Mouse | Rabbit |
|
| Concentration: |
| ~4mg/ml |
| Immunogen: |
| Synthetic peptide corresponding to aa 17-31 (D17LKEEKDINNNVKKT31) of mouse inducible nitric oxide synthase (iNOS; NOS II) |
| Purity: |
| Purified |
| Form |
| Liquid. Purified IgG in PBS containing 1mg/ml BSA and 0.05% sodium azide |
| Specificity: |
| Recognizes human, mouse and rat iNOS (NOS II). Detects a band of ~135kDa by Western blot. Does not cross-react with eNOS (NOS III) or nNOS (NOS I). |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
Upregulation of nitric oxide synthase in mice with severe hypoxia-induced pulmonary hypertension: K.A. Fagan, et al.; Respir. Res. 2, 306 (2001) |
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