Technical Data
I0800-07M
Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)
Description:
Applications:
Suitable for use in ELISA, Western Blot amd Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:3000
Immunocytochemistry: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabSerum
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
hiNOS (Cys1137-1138-1153) covalently attached onto a carrier protein.
Purity:
Serum
Form
Supplied as a lyophilized powder. Reconstitution with 0.1ml of 10mg/ml BSA in PBS.
Specificity:
Recognizes hiNOS (1137-1153) and recombinant hiNOS (NOS type II). Does not react to hnNOS (1411-1433), recombinant hnNOS (NOS type I), heNOS (1182-1203) and recombinant heNOS (type III). Specific for the carboxyl end of hiNOS and is suitable for immunocytochemical and Western immunoblot detection of hiNOS.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.