Technical Data
I0800-08
Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)
Description:
Nitric oxide (NO), a diffusible free radical gas, acts as a neurotransmitter in brain and peripheral nervous system. It accounts for the activity of endothelium-derived relaxing factors, which stimulate vasodilatation by releasing NO from the endothelium. Unlike typical neurotransmitter, NO is a not stored in synaptic vesicle and does not act on membrane receptors. Synthesis of NO, initially demonstrated in vascular endothelium, is now found in many tissues.

NO is synthesized by L-arginine, oxygen, and NADPH by three known isoforms of heme-containing flavoproteins termed NO synthase (NOS, I-III, mol wt. ~130-160kD). One group of enzyme is constitutive, agonist-triggered, and dependent on Ca2+/Calmodulin and is inhibited by L-arginine analogues (L-NNA, L-NMMA). It is found in endothelium, adrenal glands, brain and platelets. The other principle group is inducible, Ca2+/Calmodulin-independent, and inhibited by NMMA and L-NNA. It has been found in macrophage, hepatocytes, tumor cells, vascular smooth muscle and endothelial cells. Analyses of cDNA clones have identified three distinct NOS genes in mammals: neuronal (nNOS/bNOS/NOS-I), endothelial (eNOS/NOS-III), and macrophage (mNOS/iNOS/NOS-II). Both nNOS and eNOS are constitutive and the mNOS/iNOS is inducible. Sequence homology among different cloned isoforms is ~ 50%.

Applications:
Suitable for use in ELISA, Western Blotting. Other applications not tested.

Recommended Dilution:
ELISA: Control peptide should be coated at 1ug/ml.
Western Blotting: 1:100-1:1000. Recognizes 130kD protein.
Immunohistochemistry: Not recommended.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Control peptide: Catalog# I0800-09A
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceMouseRabbit
Concentration:
As reported
Immunogen:
C-terminal 19 amino acid peptide from mouse macrophage nitric oxide synthase. Human, rat and mouse iNOS/NOS-2 are ~1145aa proteins
Purity:
Serum
Form
Supplied as a liquid with 0.1% sodium azide, 40% glycerol.
Specificity:
Recognizes mouse inducible nitric oxide synthase (iNOS, NOS-II). Sequence is 84% conserved in human and rat (16/19 aa;). No significant sequence homology is seen with NOS-1/NOS-3 or other proteins. Antibody crossreactivity in various species is not established.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lowenstein CJ et al (1992) PNAS 89, 6711-6715; Lyons CR et al (1992) J Biol Chem. 267, 6370-6374; Zie Q-W et al (1992) Science 256, 225-228; Nunokawa Y et al (1993) BBRC 191, 89-94