Technical Data
I0800-08K
Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)
Description:
Nitric oxide (NO), a diffusible free radical gas, acts as a neurotransmitter in brain and peripheral nervous system. It accounts for the activity of endothelium-derived relaxing factors, which stimulate vasodilatation by releasing NO from the endothelium. Unlike typical neurotransmitter, NO is a not stored in synaptic vesicle and does not act on membrane receptors. Synthesis of NO, initially demonstrated in vascular endothelium, is now found in many tissues.

Applications:
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Immunofluorescence: 1:20
Western Blot: 1:10, detects a band at ~130kD
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k7C229Supernatant
SizeStorageShippingSourceHost
1ml-20CBlue IceHumanMouse
Concentration:
Not Determined
Immunogen:
Recombinant human iNOS.
Purity:
Supernatant
Form
Supplied as a liquid.
Specificity:
Recognizes human iNOS (aa1009-1026) and recombinant human iNOS (Type II). Does not react to heNOS (aa1040-1057), rheNOS (Type III), hnNOS (aa1280-1297) and rhnNOS (Type I). It has been epitope mapped using 96 overlapping 18 amino acid long synthetic peptides which cover the entire 1153aa length of hiNOS and was found to bind to a defined region of the hiNOS sequence, aa1009-1026.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.