		Technical Data
I0800-08K	Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)

Description:
Applications:
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:20
Western Blot: 1:10, detects a band at ~130kD
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1,k	7C229	Supernatant

Size		Storage	Shipping	Source	Host
1ml		4°C (-20°C Glycerol)	Blue Ice	Human	Mouse

Concentration:

Not determined

Immunogen:

Recombinant human iNOS.

Purity:

Supernatant

Form

Supplied as a liquid.

Specificity:

Recognizes human iNOS (aa1009-1026) and recombinant human iNOS (Type II). Does not react to heNOS (1040-1057), rheNOS (Type III), hnNOS (1280-1297) and rhnNOS (Type I). It has been epitope mapped using 96 overlapping 18 amino acid long synthetic peptides which cover the entire 1153aa length of hiNOS and was found to bind to a defined region of the hiNOS sequence, aa1009-1026.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.