Technical Data
I0800-08Q
Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)
Description:
Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:8
Optimal dilutions to be determined by the researcher.

Control Peptide:
I0800-08Q1: Inducible Nitric Oxide Synthase, Control Peptide (iNOS, iNOS 2, mNOS); 150nM. Reconstitute with 600ul stock antibody solution for blocked antibody control.

Recommended Positive Control:
A-172 cells

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, TBS/TweenŽ-20, 1% normal goat serum. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
5ml4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Peptide analog of the carboxyl terminal of hiNOS covalently attached onto a carrier protein.
Purity:
Purified by precipitation, dialysis and chromatography.
Form
Supplied as a lyophilized powder. Reconstitution: Reconstitute with 5ml of sterile 40-50% glycerol, TBS/TweenŽ-20, 1% normal goat serum.
Specificity:
Recognizes hiNOS (1137-1153). The reactivity of rhiNOS (Type 2) is ~50%. Does not react to rhnNOS (Type 1) or rheNOS (Type 3). Has been found to stain specifically hiNOS in Western immunoblots of A-172 cells induced to produce hiNOS.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.