Technical Data
I0800-09
Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)
Description:
Nitric oxide (NO), a diffusible free radical gas, acts as a neurotransmitter in brain and peripheral nervous system. It accounts for the activity of endothelium-derived relaxing factors, which stimulate vasodilatation by releasing NO from the endothelium. Unlike typical neurotransmitter, NO is a not stored in synaptic vesicle and does not act on membrane receptors. Synthesis of NO, initially demonstrated in vascular endothelium, is now found in many tissues.

NO is synthesized by L-arginine, oxygen, and NADPH by three known isoforms of heme-containing flavoproteins termed NO synthase (NOS, I-III, MW ~130-160kD). One group of enzyme is constitutive, agonist-triggered, and dependent on Ca2+/Calmodulin and is inhibited by L-arginine analogues (L-NNA, L-NMMA). It is found in endothelium, adrenal glands, brain and platelets. The other principle group is inducible, Ca2+/Calmodulin-independent, and inhibited by NMMA and L-NNA. It has been found in macrophage, hepatocytes, tumor cells, vascular smooth muscle and endothelial cells. Analyses of cDNA clones have identified three distinct NOS genes in mammals: neuronal (nNOS/bNOS/NOS-I), endothelial (eNOS/NOS-III), and macrophage (mNOS/iNOS/NOS-II). Both nNOS and eNOS are constitutive and the mNOS/iNOS is inducible. Sequence homology among different cloned isoforms is ~ 50%.

Applications:
Suitable for use in ELISA, Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: Control peptide should be coated at 1ug/ml.
Western Blot: 1:100-1:1000. Recognizes 130kD protein.

Optimal dilutions to be determined by the researcher.

Control Peptide: I0800-09A

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20°CBlue IceMouseRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide mapping to 14 amino acids within the C-terminal of mouse nitric oxide synthase (KLH coupled). Human, rat and mouse iNOS/NOS-2 are ~1145aa proteins.
Purity:
Serum
Form
Supplied as a liquid, serum, 0.1% sodium azide, 40% glycerol.
Specificity:
Recognizes mouse inducible nitric oxide synthase (iNOS, NOS-II). No significant sequence homology of iNOS-P is seen with NOS-1/NOS-3 or other proteins. Species homology: Human and rat (85%, 12/14 aa).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lowenstein CJ et al (1992) PNAS 89, 6711-6715. 2. Lyons CR et al (1992) J Biol Chem. 267, 6370-6374. 3. Zie Q-W et al (1992) Science 256, 225-228. 3. Nunokawa Y et al (1993) BBRC 191, 89-94.