Technical Data
I0800-09B
Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)
Description:
Nitric oxide (NO), a diffusible free radical gas, acts as a neurotransmitter in brain and peripheral nervous system. It accounts for the activity of endothelium-derived relaxing factors, which stimulate vasodilatation by releasing NO from the endothelium. Unlike typical neurotransmitter, NO is a not stored in synaptic vesicle and does not act on membrane receptors. Synthesis of NO, initially demonstrated in vascular endothelium, is now found in many tissues.

NO is synthesized by L-arginine, oxygen, and NADPH by three known isoforms of heme-containing flavoproteins termed NO synthase (NOS, I-III, MW ~130-160kD). One group of enzyme is constitutive, agonist-triggered, and dependent on Ca2+/Calmodulin and is inhibited by L-arginine analogues (L-NNA, L-NMMA). It is found in endothelium, adrenal glands, brain and platelets. The other principle group is inducible, Ca2+/Calmodulin-independent, and inhibited by NMMA and L-NNA. It has been found in macrophage, hepatocytes, tumor cells, vascular smooth muscle and endothelial cells. Analyses of cDNA clones have identified three distinct NOS genes in mammals: neuronal (nNOS/bNOS/NOS-I), endothelial (eNOS/NOS-III), and macrophage (mNOS/iNOS/NOS-II). Both nNOS and eNOS are constitutive and the mNOS/iNOS is inducible. Sequence homology among different cloned isoforms is ~ 50%.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:3000. Recognizes 130kD protein.

Optimal dilutions to be determined by the researcher.

Control Peptide:
I0800-09A

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug4°C (-20°C Glycerol)Blue IceMouseRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide mapping to 14 amino acids within the C-terminal of mouse nitric oxide synthase (KLH coupled). Human, rat and mouse iNOS/NOS-2 are ~1145aa proteins.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Specificity:
Recognizes mouse inducible nitric oxide synthase (iNOS, NOS-II). No significant sequence homology of immunizing peptide is seen with NOS-1/NOS-3 or other proteins. Human and rat peptides are 85% homologous with mouse peptide (12/14aa).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lowenstein, C.J., et al., PNAS 89: 6711-6715 (1992). 2. Lyons, C.R., et al., J. Biol. Chem. 267: 6370-6374 (1992). 3. Zie, Q-W., et al., Science 256: 225-228 (1992). 4. Nunokawa, Y., et al., BBRC 191: 89-94 (1993).