Technical Data
I0800-10
Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)
Description:
Applications:
Suitable for use in ELISA, Immunocytochemistry, Indirect Immunofluorescence, Immunohistochemistry and Western Blot. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:1000. A single band at ~130kD was detected using homogenate from cells expressing hiNOS.
Indirect Immunofluorescence: 1:1000-1:5000
Immunohistochemistry: Frozen. not recommended for paraffin sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile 40-50% glycerol, PBS. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
hiNOS (Cys1137 - 1138-1153) covalently attached to a carrier protein.
Purity:
Serum
Form
Supplied as a lyophilized powder. Reconstitute with 100ul sterile 40-50% glycerol, PBS containing10mg/ml BSA. No preservative.
Specificity:
Recognizes human Inducible Nitric Oxide Synthase (iNOS). Specific for the carboxyl end of hiNOS. Crossreactivity: hiNOS (1137-1153): 100%, recombinant hiNOS (NOS type II): 100%, hnNOS (1411-1433): 0%, recombinant hnNOS (NOS type I): 0%, heNOS (1182-1203): 0%, recombinant heNOS (type III): 0% Species crossreactivity: Does not crossreact with rat iNOS.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.