Technical Data
I0800-10B
Inducible Nitric Oxide Synthase (iNOS, NOS 2, mNOS)
Description:
Nitric oxide (NO), a diffusible free radical gas, affects a variety of physiological functions (2, 3). NO acts as a neurotransmitter in the brain and peripheral nervous systems. It accounts for the activity of endothelium-derived relaxing factors, which stimulate vasodilation by releasing NO from the endothelium. NO has been implicated in neurotoxicity associated with stroke and neuro-degenerative diseases, neuronal regulation of smooth muscle including peristalsis, and penile erections. In macrophages, NO mediates tumoricidal and bactericidal actions. It mediates the actions of the excitatory neurotransmitter glutamate in stimulation of cGMP levels. Unlike typical neurotransmitters, NO is not stored in synaptic vesicles and does not act on membrane receptors. Synthesis of NO, initially demonstrated in vascular endothelium, is now found in many tissues. NO is synthesized from L-arginine, oxygen, and NADPH by three known isoforms of heme-containing flavoproteins termed NO synthase (NOS I-III, mol wt. ~130160kDa). One group of enzymes is constitutive, agonist-triggered, dependent on Ca2+/calmodulin and is inhibited by L-arginine analogues (L-NNA, L-NMMA). It is found in endothelium, adrenal glands, brain, and platelets. The other principle group is inducible, Ca2+/calmodulin-independent, and also inhibited by NMMA and L-NNA. It has been found in macrophages, hepatocytes, tumor cells, vascular smooth muscle and endothelial cells. Analysis of cDNA clones has identified three distinct NOS genes in mammals: neuronal (nNOS/bNOS/NOS-I) (4), endothelial (eNOS/NOS-III) (5), and macrophage (mNOS/iNOS/NOS-II) (6). Both nNOS and eNOS are constitutive and the mNOS is inducible.

Applications:
Suitable for use in Immunoprecipitation and Western Blot. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1:1,000 (ECL). Detects a band at ~130kD.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
25ul-20CBlue IceMouseRabbit
Concentration:
Not determined
Immunogen:
A 14 residue synthetic peptide based on the mouse iNOS (residues 1131-1144) with the cysteine (C) residue added and the peptide coupled to KLH
Purity:
Purified by Protein A affinity chromatography
Form
Supplied as a liquid in PBS containing 50% glycerol and 0.09% sodium azide
Specificity:
Recognizes mouse iNOS. Species Crossreactivity: bovine, human and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Lyons, C.R., Orloff, G.J., Cunningham, J.M. (1992) J. Biol. Chem. 267: 6370-6374.
2. Marletta, M. (1994) Cell 78: 927-930.
3. Nathan, C., Xie, Q.W. (1994) Cell 78: 915-918.
4. Bredt, D.S., Hwang, P.M., Glatt, C.E., Lowenstein, C., Reed, R.R., Snyder, S.H. (1991)
Nature 351: 714-718.
5. Lamas, S. Marsden, P.A., Li, G.K., Tempst, P., Michel, T. (1992) PNAS 89: 6348-6352.
6. Xie, Q.W., Cho, H.J., Calaycay, J., Mumford, R.A., Swiderek, K.M., Lee, T.D., Ding, A.,
Troso, T., Nathan, C. (1992) Science 256: 225-228.