Technical Data
I0800-11
Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)
Description:
Nitric oxide (NO), a diffusible free radical gas, acts as a neurotransmitter in brain and peripheral nervous system. It accounts for the activity of endothelium-derived relaxing factors, which stimulate vasodilatation by releasing NO from the endothelium. Unlike typical neurotransmitter, NO is a not stored in synaptic vesicle and does not act on membrane receptors. Synthesis of NO, initially demonstrated in vascular endothelium, is now found in many tissues.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot (ECL): 1:1000-1:2500
Immunohistochemistry (ABC): 1:2500-1:6000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul-20°CBlue IceHumanRabbit
Concentration:
Not Determined
Immunogen:
Synthetic peptide corresponding to 19aa at the C-terminal from human chondrocyte nitric oxide synthase.
Purity:
Serum
Form
Supplied as a liquid, 0.05% sodium azide.
Specificity:
Recognizes human Inducible Nitric Oxide Synthase. No crossreactivity with nNOS and eNOS by Immunohistochemistry. Reacts with human glia and certain neurons in Alzheimer’s disease. Species Crossreactivity: rat ~1%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.