		Technical Data
I0800-12	Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)

Description:
Nitric Oxide Synthases are the only enzymes know to simultaneously require five bound cofactors, FAD, FMN, haem, tetrahydrobiopterin (BH4) and Ca++-Calmodulin (CAM). In mammals, there are three distinct genes for NOS, NOS I is neuronal, NOS II is cytokine inducible, and NOS III is endothelial. The enzymes exist as homodimers, each monomer consisting of two major domains: Nterminal oxygenase domain, which belongs to the class of haem-thiolate proteins, and Cterminal reductase domain, which is homologous to NADPH:P450 reductase. The interdomain linker between the oxygenase and reductase domains contains a CaMbinding sequence. Functionally NOS is an enzyme that catalyzes the stepwise conversion of the amino acid L-arginine to nitric oxide and L-citrulline. NOS activity stimulates cyclic GMP, inducing smooth muscle relaxation by increasing intracellular cGMP, which in turn inhibits calcium entry into the cell, and decreases intracellular calcium concentrations. This also stimulates a cGMP-dependent protein kinase that activates myosin light chain phosphatase, the enzyme that dephosphorylates myosin light chains, which leads to smooth muscle relaxation..

Applications:
Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000 detects INOS on 10ug of mouse liver lysates
Immunocytochemistry: 1:50,000 (Cultured cells)
Immunohistochemistry:1:5000 (ABC). Tissue sections of rat liver
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Serum

Size		Storage	Shipping	Source	Host
50ul		-20°C	Blue Ice	Mouse	Rabbit

Concentration:

Not Determined

Immunogen:

Synthetic peptide 14aa from C-terminus mouse macrophagae nitric oxide synthase.

Purity:

Serum

Form

Supplied as a liquid, 0.05% sodium azide.

Specificity:

Recognizes mouse inducible nitric oxide synthase (iNOS, NOS-II) at ~131kD. No crossreactivity with rat nNOS and eNOS by Western Blot and Immunohistochemistry. Species Crossreactivity: rat. Crossreactivity with human iNOS is ~1%.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Uttenthal LO et al., (1998) Neuronal and inducible nitric oxide synthase and nitrotyrosine immunoreactivities in the cerebral cortex of the aging rat. Microsc Res Tech 43:75–88. 2. Molina-Holgado, E. et al., European J Neuroscience (2001) 13:493–502.