Technical Data
I0800-14E
Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)
Description:
Applications:
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:20
Western Blot: 1:20
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k7C218Supernatant
SizeStorageShippingSourceHost
1ml-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Recombinant hiNOS
Purity:
Supernatant
Form
Supplied as a liquid.
Specificity:
Recognizes hiNOS (985-1002) and rhiNOS (Type II). The reactivity of heNOS (1017-1034) and rheNOS (Type III) is ~60%. Does not react to hnNOS (1256-1273) and rhnNOS (Type I). It has been epitope mapped using 96 overlapping 18 amino acid long synthetic peptides which cover the entire 1153 amino acid length of hiNOS and was found to bind to a defined region of the hiNOS sequence, residues 985-1002.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.