Technical Data
Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)
NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti-tumor and antipathogenic activities. NOS is classified under three types: Type I NOS is 150-160kD and is found in neurons. It is constitutively expressed and is Ca2+/calmodulin dependent. It is called NOS-1, neuronal NOS (nNOS), brain NOS (bNOS), cerebral/constitutive/Ca2+ regulated NOS (cNOS). bNOS is present in skeletal muscles and its absence in Duchenne’s muscular dystrophy accounts for its symptoms of the disease.
Type II NOS is 130kD protein known as macrophage NOS (mNOS) or inducible NOS (iNOS). It is Ca2+/calmodulin independent and is expressed in activated macrophages and stimulated glial cells. Type III NOS is a 135kD protein called endothelial NOS (eNOS).

Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Immunofluorescence: 1:20
Western Blot: 1:40
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
1ml-20°CBlue IceHumanMouse
Not Determined
Recombinant hiNOS
Supplied as a liquid.
Recognizes hiNOS (781-798) and rhiNOS (Type II). Does not react to heNOS (806-823), rheNOS (Type III), hnNOS (1045-1062) and rhnNOS (Type I). It has been epitope mapped using 96 overlapping 18 amino acid long synthetic peptides which cover the entire 1153 amino acid length of hiNOS and was found to bind to a defined region of the hiNOS sequence, residues 781-798.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.