Technical Data
I0800-14V
Inducible Nitric Oxide Synthase (iNOS, iNOS 2, mNOS)
Description:
Applications:
Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:180
Western Blot: 1:40
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k7C227Supernatant
SizeStorageShippingSourceHost
1ml-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Recombinant hiNOS
Purity:
Supernatant
Form
Supplied as a liquid.
Specificity:
Recognizes hiNOS (781-798) and the reactivity of rhiNOS (Type II) is ~85%. Does not react to heNOS (806-832), rheNOS (Type III), hnNOS (1045-1062) and rhnNOS (Type I). It has been epitope mapped using 96 overlapping 18 amino acid long synthetic peptides which cover the entire 1153 amino acid length of hiNOS and was found to bind to a defined region of the hiNOS sequence, residues 781-798.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.