		Technical Data
I1000	Interferon gamma, Recombinant, Mouse (IFNg)	20ug 100ug

Growth Factors, Cytokines

Storage: -20°CShipping: RT

Murine IFN-gamma is a regulatory protein produced by activated NK cells and CD4+TCR alpha-beta+, CD8+TCR alpha-beta+, and TCR gamma-delta+ T cells. Biological activities of IFN-gamma include specific binding to a single class of high affinity receptors. Recombinant murine IFNg is a 15.6kD protein containing 134 amino acid residues.

Biological Activity:
The ED50 as determined by a cytopathic effect inhibition assay with murine NIH 3T3 cells challenged with VSV virus was 0.1ng/ml corresponding to a specific activity of 1x10e7units/mg.

Amino Acid Sequence:
MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQSQII SFYLRLFEVL KDNQAISNNI SVIESHLITT FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL LPESSLRKRK RSRC

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. For extended storage it is recommended to further dilute in a buffer containing a carrier protein (0.1% BSA). Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
15.6kD

Source: E. coli
Purity: 98% by SDS-PAGE. Endotoxin: 0.1ng/ug (1EU/ug).
Concentration: ~1mg/ml
Form: Supplied as a lyophilized powder from 10mM sodium phosphate, pH 7.0, 25mM sodium chloride. Reconstitute in sodium phosphate, pH 8.0 to 1mg/ml. Further dilutions should be made into RPMI + 10% FCS or 5% HSA.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

US Biological application reference: Gardner, J.L et al., (2007) Am J Physiol Gastrointest Liver Physiol 292:G1695-G170.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.