Technical Data
I1890-05L
IgA (Biotin)
Description:
Applications:
Suitable for use in Western Blot, ELISA, and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:10,000-1:100,000, 1ug of Rat IgA in a standard capture using Streptavidin (HRP) and ABTS (A0030) as a substrate for 30 minutes at RT.
Western Blot: 1:2000-1:10,000
Immunohistochemistry: 1:1000-1:5000
Optimal dilution determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg-20°CBlue IceRatGoat
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Rat IgA alpha heavy chain
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Reconstitute with 1ml sterile ddH2O. Labeled with Biotin.
Specificity:
Recognizes rat IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-goat serum, rat IgA and rat serum. Does not cross-react with other rat heavy or light chain proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Bayer & Wilchek Methods in Enzymology 184; 138–160, 1990.