Technical Data
I1890-05M
IgA, alpha (HRP)
Description:
Applications:
Suitable for use in Western Blot, ELISA and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
ELISA: 1:150,000
Western Blot: 1:2000-1:10,000
Immunohistochemistry: 1:1000-1:5000
Optimal dilutions to be determined by the researcher

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Conjugated to horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceRatGoat
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Rat IgA, alpha chain
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% thimerasol. Labeled with HRP. Reconstitute with 1ml sterile ddH2O.
Specificity:
Recognizes rat IgA, alpha chain. Specificity was confirmed by ELISA at <1% crossreactivity against other rat heavy or light chain isotypes. Assay by IEP resulted in a single precipitin arc against anti-peroxidase, anti-goat serum, rat IgA and rat serum.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Farr & Nakane, J. Immunol. Methods 47: 129-144 (1981).