Technical Data
I1890-20C
IgA
Description:
Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and ELISA. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: (frozen) 1:10
ELISA: 1:250 (as detecting antibody)
Optimal working dilutions to be determined by researcher.

Recommended Secondary Reagents:
I1904-65W: IgG, F(ab’)2 (HRP) Pab RbxMo
I1904-66C: IgG, F(ab’)2, X-Adsorbed (HRP) Pab RbxMo

Hybridoma:
Spleen cells from immunized mice were fused with cells of the mouse P3X63-Ag8.653 cell line.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG13H2251Supernatant
SizeStorageShippingSourceHost
2ml4°C (-20°C Glycerol)Blue IcePorcineMouse
Concentration:
As reported
Immunogen:
Porcine IgA.
Purity:
Tissue culture supernatant.
Form
Supplied as a liquid, 0.1% sodium azide.
Specificity:
Recognizes porcine Ig alpha chain. No crossreactivity with IgM and IgG.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Inskeep, T. K. et al., (2010) Clin. Vaccine Immunol. doi:10.1128/CVI.00078-10.