Technical Data
I1890-20C
IgA
Description:
Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and ELISA. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry: (frozen) 1:10
ELISA: 1:250 (as detecting antibody)
Optimal working dilutions to be determined by researcher.

Recommended Secondary Reagents:
I1904-65W: IgG, F(ab)2 (HRP) Pab RbxMo
I1904-66C: IgG, F(ab)2, X-Adsorbed (HRP) Pab RbxMo

Hybridoma:
Spleen cells from immunized mice were fused with cells of the mouse P3X63-Ag8.653 cell line.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG13H2251Supernatant
SizeStorageShippingSourceHost
2ml4C (-20C Glycerol)Blue IcePorcineMouse
Concentration:
As reported
Immunogen:
Porcine IgA.
Purity:
Tissue culture supernatant.
Form
Supplied as a liquid, 0.09% sodium azide.
Specificity:
Recognizes porcine Ig alpha chain. No crossreactivity with IgM and IgG.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Inskeep, T. K. et al., (2010) Clin. Vaccine Immunol. doi:10.1128/CVI.00078-10.