Technical Data
I1890-21A
IgA (Texas Red)
Description:
IgA is useful in the identi cation of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid in ltrates indicates that the in ltrate is clonal and therefore malignant.

Applications:
Suitable for use in ELISA and Immunofluorescence. Other applications not tested.

Recommended Dilution:
ELISA: 1:10,000-1:50,000
Immunofluorescence Microscopy: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.

Absorption Wavelength: 596nm

Emission Wavelength: 620nm

Fluorochrome/Protein Ratio:
3.0 moles Texas Red per mole of Rabbit IgG

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Texas Red® conjugates are sensitive to light.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
1mg-20°CBlue IceMouseRabbit
Concentration:
~1mg/ml
Immunogen:
Mouse IgA heavy chain
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Texas Red™ Sulfonyl Chloride (MW: 625 daltons). Reconstitute with 1ml sterile ddH2O.
Specificity:
Recognizes mouse IgA. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, mouse IgA and mouse serum. Specificity was confirmed by ELISA at <1% against other mouse heavy or light chain combinations.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. J.A. Titus, P.P. Haugland, S.D. Sharrow, D.M. Segal J. Immunol. Methods 50; 193, 1982.