Technical Data
I1890-27A
IgA (HRP)
Description:
Applications:
Suitable for use in ELISA and Immunoprecipitation. Other applications have not been tested.

Recommended Dilutions:
Immunoprecipitation: Reported to immunoprecipitate bands of ~62kD and 75kD, which is consistent with the heavy chain and the secretory component of IgA (1).
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG2aIL-A71Affinity Purified
SizeStorageShippingSourceHost
250ug-20CBlue IceBovineMouse
Concentration:
~1mg/ml
Immunogen:
Purified bovine Immunoglobulin
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.01% thiomersal. Labeled with HRP.
Specificity:
Recognizes bovine IgA.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Williams, D.J.L. et al. (1990) Quantitation of bovine immunoglobulin isotypes and allotypes using monoclonal antibodies. Vet. Immunol. Immunopathol. 24: 267-283. 2. van Dissel, J.T. et al. (2005) Bovine antibody-enriched whey to aid in the prevention of a relapse of Clostridium difficile-associated diarrhoea: preclinical and preliminary clinical data. J. Med. Microbiol. 54: 197-205.