Technical Data
I1900-01H
IgE (Biotin)
Description:
Applications:
Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilutions:
ELISA: 500ng/ml as detecting antibody
Optimal working dilutions to be determined by researcher.

Hybridoma:
Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG13H2277Affinity Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Rat IR162, IR1016, IR2 and IR410 IgE myeloma proteins.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol. Labeled with Biotin.
Specificity:
Recognizes the epsilon chain of rat IgE and does not crossreact with other classes of rat immunoglobulin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bazin, H., et al., J. Immunol. 121: 2077-2081 (1987). 2. Bazin, H., et al., J. Imm. Methods 71: 9-16 (1984). 3. Bazin, H., et al., J. Imm. Methods 66: 261-269 (1984). 4. Negrao-Cooea, D., et al., J. Immunol. 157: 4037-4044 (1996). 5. Bazin, H., et al., Immunology 26: 713-723 (1974).