Suitable for use in ELISA and Western Blot. Other applications have not been tested.
ELISA: 500ng/ml as detecting antibody
Optimal working dilutions to be determined by researcher.
Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|500ug||4°C (-20°C Glycerol)||Blue Ice||Rat||Mouse|
|Rat IR162, IR1016, IR2 and IR410 IgE myeloma proteins.|
|Purified by immunoaffinity chromatography.|
|Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol. Labeled with Biotin.|
|Recognizes the epsilon chain of rat IgE and does not crossreact with other classes of rat immunoglobulin. |
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Bazin, H., et al., J. Immunol. 121: 2077-2081 (1987). 2. Bazin, H., et al., J. Imm. Methods 71: 9-16 (1984). 3. Bazin, H., et al., J. Imm. Methods 66: 261-269 (1984). 4. Negrao-Cooea, D., et al., J. Immunol. 157: 4037-4044 (1996). 5. Bazin, H., et al., Immunology 26: 713-723 (1974).|