Description:
Applications:
Suitable for use in ELISA and Western Blot. Other applications have not been tested.
Recommended Dilution:
ELISA: 500ng/ml as detecting antibody
Optimal working dilutions to be determined by researcher.
Hybridoma:
Spleen cells from immunized BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|
| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG1 | 3H2277 | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 500ug | | 4°C (-20°C Glycerol) | Blue Ice | Rat | Mouse |
|
| Concentration: |
| ~1mg/ml |
| Immunogen: |
| Rat IR162, IR1016, IR2 and IR410 IgE myeloma proteins. |
| Purity: |
| Purified by immunoaffinity chromatography. |
| Form |
| Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol. Labeled with Biotin. |
| Specificity: |
| Recognizes the epsilon chain of rat IgE and does not crossreact with other classes of rat immunoglobulin. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Bazin, H., et al., J. Immunol. 121: 2077-2081 (1987). 2. Bazin, H., et al., J. Imm. Methods 71: 9-16 (1984). 3. Bazin, H., et al., J. Imm. Methods 66: 261-269 (1984). 4. Negrao-Cooea, D., et al., J. Immunol. 157: 4037-4044 (1996). 5. Bazin, H., et al., Immunology 26: 713-723 (1974).
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