Technical Data
I1900-01J
IgE (FITC)
Description:
Applications:
Suitable for use in Flow Cytometry. Other applications have not been tested.

Recommended Dilution:
Flow Cytometry: 5ug/ml. Use 50ul to label 2 x 10e7 cells in100ul sample volume.
Optimal dilution to be determined by the researcher.

Hybridoma: Spleen cells from an immunized BALB/c mouse fused with mouse SP2/0 myeloma cells

Recommended Negative Controls: Mouse IgG1 Negative Control: FITC

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.




TypeIsotypeCloneGrade
MabIgG13H2277Affinity Purified
SizeStorageShippingSourceHost
500ug-20°CBlue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Rat IgE myeloma proteins (IR162, IR1016, IR2 and IR410)
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, containing 0.1% sodium azide and 50% glycerol. Labeled with FITC.
Specificity:
Specific for the Epsilon chain of rat immunoglobulin IgE and does not cross-react with other classes of rat immunoglobulin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bazin, H., Querinjean, P., Beckers, A., Heremans, J.F. and Dessy, F. (1974). Immunology 26: 713-723.



2. Bazin, H., Beckers, A., Urbain-Vansanten, G., Pauwels, R., Bruyns, C., Tilkin, A.F., Plateau, B. and Urbain, J. (1978). J. Immunol. 121: 2077